WebTHE JOURNAL OFBIOLOOICAL CHEMISTRY Vol. 239, No. 9, September 1964 Printed in U.S.A. Re-formation of Interchain Bonds Linking Half-molecules of Rabbit y-Globulin* $3. R. STEIN, J. L. PALMER, AND A. NISONOFF~ From the Department of Microbiology, University of Illinois, Urbana, Illinois (Received for publication, March 13, 1964) It was recently ... Webflodin, p, fractionation of human-serum proteins by gel filtration ... porath, j, migration-elution of zones in an electrophoresis column, nature 182: 744 (1958). google scholar. porath, j, gel filtration - method for desalting and group separation, nature 183: 1657 (1959). google scholar. porath, j, separation methods based on molecular ...
Size Exclusion Chromatography Springer Nature Experiments
WebHowever, at low concentrations of cystine (0.002 to 0.01 InM) the cells synthesized 16 to 80% of their cystine residues de nova, the cystine carbon deriving from glucose and the sulfur from methionine (Table I). Still lower concentrations of cystine were ineffective. As illustrated in the experiment of Table II, this requirement for exogenous ... WebSize-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers.Typically, when an aqueous solution … citizen golf watch
The Biosynthesis of Cystine in Human Cell Cultures
WebThe usefulness of a new polyacrylamide gel of small pore size for the desalting of proteins and peptides has been demonstrated. It has also been shown that neutral amino acids can be partially desalted. Maximum desalting of small molecules is obtained when a slow flow rate and a small particle size of gel are used. WebJun 1, 2009 · A method was devised for separating proteins of skim-milk on Sephadex G 100 dextran gel. Four fractions were obtained, the first 3 corresponding to casein micelle, lactoglobulin, and lactalbumin fractions while the last contained no protein. WebPorath and Flodin ascribe this phenomenon to a form of molecular sieving, ... Porath, J., and Flodin, P., Nature, 183, 1657 (1959). Article ADS CAS Google Scholar ... citizen golf watch replacement band